Coding

Part:BBa_K3426000:Design

Designed by: Kyra Eyerman   Group: iGEM20_UCSC   (2020-10-21)


Cellulose-Binding Modules of Type 2a (CBM2a) Fused to mRFP1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 361
    Illegal PstI site found at 910
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 361
    Illegal PstI site found at 910
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 361
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 361
    Illegal PstI site found at 910
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 361
    Illegal PstI site found at 910
    Illegal AgeI site found at 2247
    Illegal AgeI site found at 2359
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since this particular CBM2a sequence is 1458bp, we ordered the mFRP and CBM2a as 2 separate gene blocks from IDT.



Source

The genomic sequence for CBM2a was listed on NCBI GenBank (GenBank: HQ445939.1). The sequence for mRFP/C and N terminus linkers were derived from Part:BBa_K2668020. IDT assembled gene blocks and primers.

References